Product of the month: May
This May at a special price at neoFroxx: IPTG for biochemistry.
Isopropyl-β-D-thiogalactopyranoside (IPTG) is indispensable in cloning experiments with E. coli. As an artificial activator of the lactose operon, IPTG ensures that the expression of genes can be specifically switched on. Therefore recombinant proteins can be produced instantly. The gene of interest only needs to be cloned into a suitable plasmid so that it is under the control of a lac repressor-regulated promoter.
The advantage of IPTG is that it is not metabolised by the bacteria and its concentration remains constant during expression – in contrast to the natural activator of the lac operon, lactose or allolactose.
IPTG stock solutions (e.g. 1 M IPTG in water; sterile filtered through 0.2 μm filter) are stable for up to six months when stored in deep freeze. The working concentration in the medium is usually between 0.2 mM and 1.0 mM.
Our IPTG for biochemistry is of plant origin and is produced by fermentation.